Sensory Characteristics, Nutrient Content, and Microbial Load of Rice Eel (Monepterus albus) Tocino, Flavored With Fruit Extracts
DOI:
https://doi.org/10.21776/ub.jpa.2025.013.02.2Keywords:
Monopterus albus, Nutrient analysis, Philippines, Rice eel, Value-added productsAbstract
This study aimed to evaluate the sensory characteristics, nutrient content, and microbial load levels of Rice eel tocino flavored with different fruit extracts. Four experimental fish tocino were formulated to contain O. niloticus + 0%fruit extract (C0), M. albus + 2.26% Philippine lime (T1), M. albus + 2.26% Lemon (T2), and M. albus + 2.26% Orange (T3). Sensory evaluation and descriptive analysis were used for sensory characteristics. FDA standards and aerobic plate count were used for nutrient content and microbial analysis. All treatments were done in triplicate and were subjected to ANOVA with a post hoc test. Results show that sensory attributes in all treatments were perceived as “liked very much”. Nutrient analysis in all formulated treatments has shown an acceptable protein content of 26%-30%. Total fat ranged from 0%-3%, while total carbohydrates ranged from 2%-4.7%. All treatment groups showed acceptable limits of microbial levels (<106 cfu/g) until 32 days of storage.
References
Aberoumand, A. (2014). Preliminary studies on nutritive and organoleptic properties in processed fish fillets obtained from Iran. Food Science and Technology, 34, 287-291.
Addo-Preko, E., Amissah, J. G. N., & Adjei, M. Y. B. (2023). The relevance of the number of categories in the hedonic scale to the Ghanaian consumer in acceptance testing. Frontiers in Food Science and Technology, 3, 1071216.
Alemu, T. (2023). Texture profile and design of food product. J. Agric. Horti. Res, 6, 272-281.
Andrews, W. (1992). Manual of food quality control. 4. Rev. 1. Microbiological analysis. Food and Drug Administration. FAO Food and Nutrition paper, 14(4 Revis 1), 1-338.
Arifan, F., Winarni, S., Wahyuningsih, W., Pudjihastuti, I., & Broto, R. W. (2019, October). Total plate count (TPC) analysis of processed ginger on Tlogowungu village. In International Conference on Maritime and Archipelago (ICoMA 2018) (pp. 377-379). Atlantis Press.
Azanza, M. P. V., & Rustia, A. S. (2004). Residual nitrite levels in Philippine sweet bacon. Food control, 15(5), 385-389.
Balachandran, K. K. (2001). Post-harvest technology of fish and fish products. Daya Books.
Bezbaruah, G., & Deka, D. D. (2021). Variation of moisture and protein content in the muscle of three catfishes: A comparative study. International Journal of Fisheries and Aquatic Studies, 9(1), 223-226.
Bursac Kovacevic, D., Barba, F. J., Lorenzo, J. M., Rocchetti, G., Lucini, L., & Putnik, P. (2019, September). Innovative technologies for fruit extracts: Value-added opportunities in the meat industry. In IOP Conference Series: Earth and Environmental Science (Vol. 333, No. 1, p. 012017). IOP Publishing.
Centre for Food Safety. (2014). Microbiological Guidelines for Food (For ready-to-eat food in general and specific food items). In Food and Environmental Hygiene Department, Hong Kong.
Chang, L., Lin, S., Zou, B., Zheng, X., Zhang, S., & Tang, Y. (2021). Effect of frying conditions on self-heating fried Spanish mackerel quality attributes and flavor characteristics. Foods, 10(1), 98.
Cheong, M. W., Zhu, D., Sng, J., Liu, S. Q., Zhou, W., Curran, P., & Yu, B. (2012). Characterisation of calamansi (Citrus microcarpa). Part II: Volatiles, physicochemical properties and non-volatiles in the juice. Food chemistry, 134(2), 696-703.
Chen H., Liu H., Li R., Lin X., Luo H., Ji H., Hu W., Luo D., 2021 Blood cell identification and hematological analysis during natural sex reversal in rice field eel (Monopterus albus). Aquaculture 538:736543.
Dash, K. K., Fayaz, U., Dar, A. H., Shams, R., Manzoor, S., Sundarsingh, A., ... & Khan, S. A. (2022). A comprehensive review on heat treatments and related impact on the quality and microbial safety of milk and milk-based products. Food Chemistry Advances, 1, 100041.
Dini, I., & Laneri, S. (2021). Spices, condiments, extra virgin olive oil and aromas as not only flavorings, but precious allies for our wellbeing. Antioxidants, 10(6), 868.
Ehsanur Rahman, S. M., Islam, S., Pan, J., Kong, D., Xi, Q., Du, Q., ... & Han, R. (2023). Marination ingredients on meat quality and safety—A review. Food Quality and Safety, 7, fyad027.
Erkan, N., Ozden, O., & Selcuk, A. (2010). Effect of frying, grilling, and steaming on amino acid composition of marine fishes. Journal of Medicinal Food, 13(6), 1524-1531. PMid:20874243. http://dx.doi. org/10.1089/jmf.2009.0203
Ekawati, E. R., & Darmanto, W. (2019). Lemon (Citrus limon) juice has antibacterial potential against diarrhea-causing pathogen. In IOP conference series: Earth and environmental science (Vol. 217, No. 1, p. 012023). IOP Publishing
Espejo-Hermes, J. (2004). Fish processing technology in the tropics (2nd ed.). TAWID Publications
Fadhlullah, M., Prasetyati, S. B., & Marleni, N. R. (2021, February). The application of different heat processing technique on eel (Monopterus albus) galantine. In IOP Conference Series: Earth and Environmental Science (Vol. 674, No. 1, p. 012003). IOP Publishing.
Guerrero III, R. D. (2014). Impacts of introduced freshwater fishes in the Philippines (1905-2013): A review and recommendations. Philippine Journal of Science, 143(1), 49-59.
Guan, R. Z., L. H. Zhou, G. H. Cui, X. J. Feng. 1996. Studies on the artificial propagation of Monopterus albus (Zuiew). Aquaculture Research 1996:587-596.
Heymann, H., & Lawless, H. T. (2013). Sensory evaluation of food: principles and practices. Springer Science & Business Media.
Islam, M. A., Mohibbullah, M., Suraiya, S., Sarower‐E‐Mahfuj, M., Ahmed, S., & Haq, M. (2020). Nutritional characterization of freshwater mud eel (Monopterus cuchia) muscle cooked by different thermal processes. Food Science & Nutrition, 8(11), 6247-6258.
Juliano R.O., Guerrero III R. & Ronquillo I. (1989). The introduction of exotic aquatic species in the Philippines, p. 83-90. In: De Silva, S.S. (ed.) Exotic aquatic organisms in Asia. (19-21 June 1981) Proceedings of the Workshop on Introduction of Exotic Aquatic Organisms in Asia. Asian Fish. Soc. Spec. Publ. 3, 154 p.
Lubis, N. D. A., Ichwan, M., & Nasution, I. K. (2020). Introducing Eel Nugget as a Potential Alternative of High-protein Processed Food.
Manalo, M., & Benedicto, A. (2014, March). Effects of different humectants in the physico-chemical properties of ready-to-eat (RTE) tocino. In DLSU Research Congress.
Manner, I. H., Buker, R. S., Smith, V. E., Ward, D. and Elevitch, R. C. (2006) Species Profiles for Pacific Island Agroforestry www.traditionaltree.org.
Mapatac, L. C. (2015). Antibacterial, Histochemical and Phytochemical Screening and Cytotoxicity Activity of Tubog, Ficus nota (Blanco) Merr Leaf and Fruit Extracts. Recoletos Multidisciplinary Research Journal, 3(2).
Matsumoto S., Kon T., Yamaguchi M., Takeshima H., Yamazaki Y., Mukai T., Kuriiwa K., Kohda M., Nishida M., (2010). Cryptic diversification of the swamp eel Monopterus albus in East and Southeast Asia, with special reference to the Ryukyuan populations. Ichthyological Research 57(1):71-77.
Maturin, L., & Peeler, J. T. (2001). BAM: Aerobic plate count. US Food and Drug Administration: Silver Spring, MD, USA, 1-10.
Milan, L. B., Feliciano, A. F., & Lusong, A. N. (2021). Food Safety Compliance and Challenges of Micro Food Business Operators: Implications to COVID-19 Pandemic. Recoletos Multidisciplinary Research Journal, 9(2), 89-102.
Muniz, V. R. G. D. F., Ribeiro, I. S., Beckmam, K. R. L., & Godoy, R. C. B. D. (2023). The impact of color on food choice. Brazilian Journal of Food Technology, 26, e2022088.
Nitura, K. P. M. D (2016). A market acceptability study of rice eel longaniza from asian swamp eel (Monopterus albus) in Aurora, Cauayan City, and San Mateo, Isabela.
Ostonal, J. M., Arugay, M. B., Bassig, B. T., Iñigo, H. B. R., Mahmudah, N. A., Dichoson, H. K. E., ... & Cabajes, A. T. S. Effects of Pre-treatment and Addition of Calamansi Juice Extract on Oyster Mushroom Kropek Sensory and Physicochemical Properties.
Padeniya, U., Larson, E. T., Septriani, S., Pataueg, A., Kafui, A. R., Hasan, E., ... & Brown, C. L. (2022). Probiotic treatment enhances pre‐feeding larval development and early survival in zebrafish Danio rerio. Journal of Aquatic Animal Health, 34(1), 3-11.
Penarubia, O., Toppe, J., Ahern, M., Ward, A., & Griffin, M. How value addition by utilization of tilapia processing by-products can improve human nutrition and livelihood, Rev. Aquacult. 15 (2023) 32–40.
Pfledderer, C. D., Ranjit, N., Pérez, A., Malkani, R. I., Ferreira De Moraes, A. C., Hunt, E. T., ... & Hoelscher, D. M. (2024). Using the Nutrition Facts Label to Make Food Choices Is Associated with Healthier Eating among 8th and 11th-Grade Students: An Analysis of Statewide Representative Data from the 2019–2020 Texas School Physical Activity and Nutrition Survey. Nutrients, 16(2), 311.
Refai, M. K. (1979). Manuals of food quality control. 4: Microbiological analysis.
Reyes, D. A. Product development and acceptability of bottled Big-eyed Scad Selar crumenophthalmus in Spanish-oil style with organic flavoring.
Richa, R., Kohli, D., Vishwakarma, D., Mishra, A., Kabdal, B., Kothakota, A., ... & Naik, B. (2023). Citrus fruit: Classification, value addition, nutritional and medicinal values, and relation with pandemic and hidden hunger. Journal of Agriculture and Food Research, 14, 100718.
Romero, J. (2020). Investigating the flavor of fresh calamondin peel and juice using instrumental and descriptive sensory analysis (Master's thesis, Texas Woman's University).
Saini, R. K., Ranjit, A., Sharma, K., Prasad, P., Shang, X., Gowda, K. G. M., & Keum, Y. S. (2022). Bioactive compounds of citrus fruits: A review of composition and health benefits of carotenoids, flavonoids, limonoids, and terpenes. Antioxidants, 11(2), 239.
Sharif, M. K., Butt, M. S., Sharif, H. R., & Nasir, M. (2017). Sensory evaluation and consumer acceptability. Handbook of food science and technology, 10, 362-386.
Suárez-Medina, M. D., Sáez-Casado, M. I., Martínez-Moya, T., & Rincón-Cervera, M. Á. (2024). The effect of low temperature storage on the lipid quality of fish, either alone or combined with alternative preservation technologies. Foods, 13(7), 1097.
Temgire, S., Borah, A., Kumthekar, S., & Idate, A. (2021). Recent trends in ready to eat/cook food products. Pharma Innov. J, 10(5), 211-217.
Tiencheu, B., Nji, D. N., Achidi, A. U., Egbe, A. C., Tenyang, N., Ngongang, E. F. T.,...& Fossi, B. T. (2021). Nutritional, sensory, physico-chemical, phytochemical, microbiological and shelf-life studies of natural fruit juice formulated from orange (Citrus sinensis), lemon (Citrus limon), Honey and Ginger (Zingiber officinale). Heliyon, 7(6).
Towers, L. (2014). Rice eel turned from pest to profit. The Fish Site.
Ünlüsayın, M., Kaleli, S., & Gulyavuz, H. (2001). The determination of flesh productivity and protein components of some fish species after hot smoking. Journal of the Science of Food and Agriculture, 81(7), 661-664.
Vivar, H. (2021). Development of Homemade Sweetened Style Bangus. Available at SSRN 3802930.
Wichchukit, S., & O'Mahony, M. (2015). The 9‐point hedonic scale and hedonic ranking in food science: some reappraisals and alternatives. Journal of the Science of Food and Agriculture, 95(11), 2167-2178.
Yang, D., Chen, F., & Ruan, G. (2018). Aquaculture of the paddy eel, Monopterus albus. Aquaculture in China: Success stories and modern trends, 283-296.
Zalameda, E.C., Falco, M.E.M., Conoza, V.B., Esteban, L.R., & Florendo, N.E.C..Preparation & preservation of fruit juices 3rd ed. (2013). ISSN 1656-6831. Livelihood Technology Series 3. Industrial Technology Development Institute-DOST.
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